Streptozotocin is a glucosaminenitrosourea compound. Read streptozotocin induced diabetes in the chinese hamster, diabetologia on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The histological changes in the islets were similar to those described after the injection of alloxan. Hyperglycemiainduced oxidative stress is a common phenomenon in diabetes. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis. Collectively, the results show that stzinduced diabetes results in a decrease in blood. Stz induces diabetes mellitus in laboratory animals. We hypothesized that the fibrosispromoting and hypertrophic actions of stat3 are linked to the activation by epidermal growth factor receptor egfr. Stz is well known to cause pancreatic bcell damage, whereas na is administered to rats to partially protect insulinsecreting cells against stz. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Dec 18, 2007 figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected.
Diabetes may lead to the development of osteoporosis. Read streptozotocininduced diabetes in the chinese hamster, diabetologia on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Female spraguedawley rats aged approximately 55 days were treated with increasing doses of stz 65, 120, or 175 mgkg ip and killed 48 hr later. The effect of streptozotocininduced diabetes in rats. Detailed in this unit are protocols for producing stz. Streptozotocininduced diabetes mellitus an overview. The effects of streptozotocin stzinduced diabetes on rat hepatic hydroxysteroid sulfotransferasea hsta and aryl sulfotransferase iv astiv gene expression were characterized.
Which type of diabetes will be induced by streptozotocin. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. Full text streptozotocininduced type 1 diabetes in rodents as a. Read effects of streptozotocininduced diabetes on phosphoglyceride metabolism of the rat liver, lipids on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. May 19, 2014 type 2 diabetes is increasing in prevalence worldwide 1, 2, and it is strongly associated with obesity and insulin resistance 3, 4, as well as defects in pancreatic cell function and mass 5, 6. Streptozotocininduced diabetes mellitus in pigs university. Given the high morbidity rate after pancreatectomy, streptozotocin should be the method of choice for inducing diabetes mellitus in pigs. Figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected. Antioxidant activity of polyherbal formulation on streptozotocin induced diabetes in experimental animals sajeeth c. Coffee drinking, apart from its health benefits, is taken into consideration as an osteoporosis risk factor. Biomimetic carbon monoxide nanogenerator ameliorates streptozotocin induced type 1 diabetes in mice.
Usually we consider the chemically induced diabetes for experimental purpose to evaluate the antidiabetic activity of herbal drugs and stz is preferred, but the mechanism of type of diabetes is. The mechanisms of alloxan and streptozotocininduced diabetes. The purpose of this study was to investigate homocysteine metabolism in a type 1 diabetic animal model and to examine whether insulin plays a role in its regulation. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Since oxidative stress depletes adiponectin and insulin levels, we investigated whether an upregulated heme oxygenase ho system would attenuate the oxidative destruction of adiponectininsulin and improve insulin sensitivity and glucose metabolism in streptozotocin stzinduced type 1 diabetes. The synthesis and secretion of proglucagonderived peptides are regulated in a tissuespecific manner in pancreas, intestine, and brain. Streptozotocininduced diabetes in the chinese hamster. Diabetes mellitus dm is a chronic disease characterized by hyperglycemia resulting in insulin resistance andor insulin secondary deficiency caused by the. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. Therefore, the aims of the present study were to assess 1 the effects of glucose and insulin on vsmc proliferation and migration in vitro, 2 response to balloon injury in rats with streptozotocin stzinduced diabetes with or without adjunct insulin therapy, and 3 neointimal hyperplasia after vascular injury in nondiabetic rats with. Hypoglycemia induced by an injection of insulin or hyperglycemia induced by. We observed a 30% reduction in plasma homocysteine in the untreated diabetic rat. In conclusion, stz induced diabetes in pigs fulfils the requirements for a good animal model for type 1 diabetes with respect to clinical signs of the disease and alterations in the carbohydrate and amino acid metabolism. High dose stz induces necrosis and multiple lowdose causes apoptosis in.
A single injection of streptozotocin into mice produced an extensive necrosis of the beta cells resulting in permanent diabetes. We tested this hypothesis by challenging cultured cardiomyocytes to highconcentration glucose and heart tissues of streptozotocin stzinduced type 1 diabetic mice. Streptozotocin stz given intravenously destroys pancreatic beta cells and is widely used in animal models to mimic type 1 diabetes. Data from human and animal studies on coffee and caffeine bone effects are inconsistent. Three days after the stz injection, the blood was withdrawn from the tail vein, and the glucose level. Transferrin tf receptormediated transcytosis of insulintransferrin conjugate in tf has been demonstrated in cultured human enterocytelike caco2 cells. Researcharticle the antidiabetic activity of nigella sativa and propolis on streptozotocininduced diabetes and diabetic nephropathy in male rats haddada. We have previously shown that in vivo there is a decrease in heart rate in the streptozotocin stz induced diabetic rats compared to controls. We tested this hypothesis by challenging cultured cardiomyocytes to highconcentration glucose and heart tissues of streptozotocin stz induced type 1 diabetic mice. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. Type 1 diabetes mellitus was induced in rats by the intraperitoneal injection of freshly prepared streptozotocin stz at a dose of 45 mgkg dissolved in 0. The longterm effects of streptozotocin and possible recovery from streptozotocin induced diabetes are explored much less. Type 2 diabetes is increasing in prevalence worldwide 1, 2, and it is strongly associated with obesity and insulin resistance 3, 4, as well as defects in pancreatic cell function and mass 5, 6.
Full text streptozotocininduced type 1 diabetes in. Therefore, the aims of the present study were to assess 1 the effects of glucose and insulin on vsmc proliferation and migration in vitro, 2 response to balloon injury in rats with streptozotocin stz induced diabetes with or without adjunct insulin therapy, and 3 neointimal hyperplasia after vascular injury in nondiabetic rats with. Sodium reten tion accompanying insulin treatment of diabetes mellitus. Sonnenblick, and james scheuer summary in order to determine whether diabetic cardiomyopathy in. Materials and methods twenty adult male albino rats were used in this study, randomly divided into three groups. Cessation of these preventive treatments was followed by the appearance of insulitis and diabetes. The effect of streptozotocininduced diabetes in rats on cardiac contractile proteins ashwani malhotra, somsong penpargkul, frederick s.
Pathophysiological mechanisms and fetal outcomes d. Sugarlowering drugs for type 2 diabetes mellitus and. Streptozotocin diabetes in the mouse and guinea pig diabetes. The effect of insulin on the contraction force in control and diabetic rats. Diabetes induced by streptozotocin at 150 mgkg body weight is complete and permanent. Hypoglycemia induced by an injection of insulin or hyperglycemia induced.
In the present report, oral delivery of insulin as a tf conjugate in streptozotocin stzinduced diabetic rats was investigated. Multiple small injections of streptozotocin in mice produce pancreatic insulitis, with progression to nearly complete beta cell destruction and diabetes mellitus. The longterm effects of streptozotocin and possible recovery from streptozotocininduced diabetes are explored much less. Changes in oxidative stress and antioxidant enzyme. The effect of streptozotocininduced diabetes in rats on. As diabetes induced by a single stz injection is often referred to as type 1.
Streptozotocin use in mice and rats version 1 date of publication. Diabetes was induced by intravenous administration of 100 mgkg streptozotocin to spraguedawley rats. Biomimetic carbon monoxide nanogenerator ameliorates. The sinus node is the pacemaker of the heart and the first component of the cardiac conduction system. Streptozotocininduced type 1 diabetes in rodents as a. Effects of streptozotocininduced diabetes and of insulin.
Tissuespecific differences in the levels of proglucagon. Effects of balloon injury on neointimal hyperplasia in. Rabbit antimouse lymphocyte serum, alone, provided partial protection but, when given together with either 3omg or nicotinamide, effectively prevented the streptozotocin induced diabetic syndrome. Administration of both streptozotocin stz and nicotinamide na has been proposed to induce experimental diabetes in the rat. The animal experiments were performed according to the guidelines for laboratory animals established by the wuhan university center for animal center experimenta3lab, and all study protocols were subjected to approval by the institutional center of wuhan university wuhan, china. The diabetic rats gained 83% less weight despite consuming 45% more feed than the nondiabetic controls. Author links open overlay panel cheng zhang siyuan peng sheng hong qiwen chen xuan zeng lei rong zhenlin zhong xianzheng zhang. A group of these mice n 12 was coinjected with recombinant trail 20. The antinociceptive potency of morphine as determined by the tailflick test was significantly decreased in streptozotocin stz induced diabetic mice and mice pretreated with hypertonic dextrose or fructose. Postmortem examination confirmed muscle wasting and a reduction of abdominal and subcutaneous fat. Which type of diabetes will be induced by streptozotocin in. Effect of streptozotocininduced diabetes mellitus on the ce. Type i diabetes, though contributes to only 510% of total diabetes cases, is a rising concern in todays world. Stzinduced diabetic rats and spontaneously diabetic mice were also significantly less sensitive to the antinociceptive effects of morphine in the tailflick test.
Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic. Stz is well known to cause pancreatic bcell damage, whereas na is ad. Retinolbinding protein rbp and transthyretin ttr in the plasma, liver and kidney, retinol in plasma, and total vitamin a in the liver were measured in rats 6 weeks after diabetes mellitus had been induced by streptozotocin stz. Streptozotocin stz induced diabetes mellitus dm offers a very cost effective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise. Full text streptozotocininduced type 1 diabetes in rodents. The effect of streptozotocin induced diabetes in rats on cardiac contractile proteins ashwani malhotra, somsong penpargkul, frederick s. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Streptozotocininduced diabetes lowers retinolbinding. The antidiabetic activity of nigella sativa propolis on. Streptozotocin diabetes iscausedbythespeci c necrosis of the pancreatic cells, and this agent is the rst. Free fulltext mdpi publisher of open access journals.
Streptozotocininduced diabetes in rats is associated with impaired metabolic availability of vitamin a retinol volume 75 issue 4 p. Heme oxygenase system enhances insulin sensitivity and. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Diabetes was induced by five consecutive daily injections of lowconcentration 50 mgkg streptozotocin stz in c57 black mice n 24. In our experiments, we followed the rats with streptozotocin induced diabetes for one year and a recovery was observed in a significant portion of animals. Review article streptozotocininduced diabetes models. The effects of streptozotocin stz induced diabetes on rat hepatic hydroxysteroid sulfotransferasea hsta and aryl sulfotransferase iv astiv gene expression were characterized.
The dependence of contraction force cf on stimulation frequency in control a and. Inhibition of egfrstat3 attenuates cardiomyopathy in. Cardiovascular disorders are common in type i diabetes mellitus t1dm for example, there is a risk of bradyarrhythmias due to dysfunction of the cardiac conduction system. Studies of streptozotocininduced insulitis and diabetes. We have previously shown that in vivo there is a decrease in heart rate in the streptozotocin stz. The aim of the study was to investigate effects of caffeine at a moderate dose on the skeletal system of rats in two models of experimental. Hypoglycemic effect of insulintransferrin conjugate in. Sonnenblick, and james scheuer summary in order to determine whether diabetic cardiomyopathy in rats is associated with altered.
The regenerative capacity of beta cells which survived the cytotoxic injury was limited. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural changes resulting in necrotic beta cell death. Stz induced diabetic rats and spontaneously diabetic mice were also significantly less sensitive to the antinociceptive effects of morphine in the tailflick test. Thus stz diabetes causes a significant decrease in cardiac contractile protein. In rats and primates, expressing a high level of glut2 expression on. We have examined the plasma and tissue levels of these peptides during the first 3 weeks of streptozotocin stzinduced diabetes in the rat. These metabolic disorders impede the critical regulatory influence of insulin on glucose, lipid and protein metabolism, thus precipitating a. Streptozotocin induced diabetes in rats is associated with impaired metabolic availability of vitamin a retinol volume 75 issue 4 p.
The antinociceptive potency of morphine as determined by the tailflick test was significantly decreased in streptozotocin stzinduced diabetic mice and mice pretreated with hypertonic dextrose or fructose. Dillman wh 1980 diabetes mellitus induces changes in cardiac myosin of the. Streptozotocin is a permanent diabetes inducing drug. Effect of streptozotocininduced diabetes mellitus on. Multiple lowdose stz mimics type i diabetes or insulindependent diabetes mellitus iddm. The timing and appearance of the inflammatory islet lesions suggest but do not prove that streptozotocin acts by initiating a cellmediated immune reaction. Back to journals diabetes, metabolic syndrome and obesity. Hyperglycemia enhances the production of reactive oxygen species, leading. We have examined the plasma and tissue levels of these peptides during the first 3 weeks of streptozotocin stz induced diabetes in the rat. Researcharticle the antidiabetic activity of nigella sativa and propolis on streptozotocin induced diabetes and diabetic nephropathy in male rats haddada. Pdf induction of diabetes by streptozotocin in rats. In our experiments, we followed the rats with streptozotocininduced diabetes for one year and a recovery was observed in a significant portion of animals. We also observed a complete loss of pp pancreatic polypeptidepositive cells. In the present study an attempt has been made to evaluate the presence of hypoglycemic activity in the alcoholic extract of aloe vera gel.
Streptozotocin stzinduced diabetes mellitus dm offers a very cost effective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible. Hypoglycemic effect of aloe vera gel on streptozotocin. Effects of streptozotocininduced diabetes on rat liver. Effect of streptozotocininduced diabetes mellitus on the. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. Diabetes mellitus and its complications are chronic glucotoxicity diseases. Streptozotocininduced type 1 diabetes in rodents as a model. Streptozotocinnicotinamideinduced diabetes in the rat. Effect of streptozotocininduced diabetes mellitus on the cerebellar cortex of adult male albino rats. Ultrastructural evidence of abundant type c viruses within beta cells of.
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